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For cells, they were first washed with PBS and fixed at room temperature for 15 min, followed by incubation with the staining solution at pH 6.0 overnight at 37 C

Check homocysteine (should be under 9) and MCV (should be 80-92) instead to detect cellular deficiency, even when serum B12 appears normal

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Peptides like Amlexanox are also being explored for their potential in managing severe chronic pain

Diagnosing this condition requires a sharp eye for these signals, and often involves blood tests that check B12 levels, a complete blood count, and antibodies related to intrinsic factor